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Molecular Genetics and Breeding of Forest Trees

Molecular Genetics and Breeding of Forest Trees by Sandeep Kumar
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  • About the Editors
  • Contributors
  • Preface
  • Part I: FOREST TREE FUNCTIONAL GENOMICS
  • Chapter 1. Functional Genomics in Forest Trees
  • Introduction
  • The Functional Genomics Tool Kit—Transcript Discovery and Profiling
  • Functional Genomics in Action
  • En Route to Functional Genomics in Forest Trees
  • Conclusions
  • Chapter 2. Expressed Sequence Tag Databases from Forestry Tree Species
  • Introduction
  • Forestry Tree EST Databases
  • The Genesis Pine and Eucalyptus EST Projects
  • Special Purpose Libraries
  • Conclusion
  • Chapter 3. Proteomics for Genetic and Physiological Studies in Forest Trees: Application in Maritime Pine
  • Introduction
  • Characterization of the Pine Proteome
  • Genetic Variability of Qualitative and Quantitative Protein Variants
  • Variability of Proteome Expression in Physiological Studies
  • Conclusion
  • Chapter 4. Exploring the Transcriptome of the Ectomycorrhizal Symbiosis
  • Introduction
  • The Ectomycorrhizal Symbiosis
  • The Anatomy and Development of Ectomycorrhiza
  • The Morphogenesis of Ectomycorrhiza
  • Alteration in Gene Expression in Ectomycorrhiza
  • The Transcriptome of Ectomycorrhizal Symbioses
  • Outlook
  • Part II: MOLECULAR BIOLOGY OF WOOD FORMATION
  • Chapter 5. Genomics of Wood Formation
  • Introduction
  • Wood Biosynthesis
  • Regulation of Wood Formation
  • Arabidopsis As a Model for the Study of Wood Formation
  • Genomics Approach to Wood Formation
  • The Outlook
  • Chapter 6. Molecular Genetics of Cellulose Biosynthesis in Trees
  • Introduction
  • Cellulose Heterogeneity in Trees
  • Cellulose Production Under Mechanical Stress
  • The Site of Cellulose Biosynthesis
  • Current Knowledge of the General Process of Cellulose Synthesis in Plants
  • Identification of CesA Genes in Plants
  • Isolation of New CesA and CSLD Genes
  • Future Perspectives
  • Chapter 7. Tuning Lignin Metabolism Through Genetic Engineering in Trees
  • Introduction
  • Genetic Modification of PAL and C4H
  • Genetic Modification of 4CL
  • Genetic Modification of C3H
  • Genetic Modification of CCoAOMT
  • Genetic Modification of CCR
  • Genetic Modification of F5H
  • Genetic Modification of COMT
  • Genetic Modification of CAD
  • Genetic Modification of Peroxidases and Laccases
  • Genetic Modification of Transcription Factors
  • Outlook
  • Chapter 8. In Vitro Systems for the Study of Wood Formation
  • Introduction
  • Cambial Activity
  • Wood Formation
  • The Formation of Tracheary Elements
  • Heartwood Formation (Tylosis and Pit Aspiration)
  • In Vitro Wood Formation Systems
  • Future Perspectives
  • Part III: FOREST TREE TRANSGENESIS
  • Chapter 9. Genetic Modification in Conifer Forestry: State-of-the-Art and Future Potential—A Case Study with Pinus radiata
  • Introduction
  • Tissue Culture As a Tool for Molecular Research
  • Genetic Engineering in Conifers
  • Modification of Traits by Suppression of Gene Expression
  • Analysis of Promoters Controlling Gene Expression in Conifers
  • Tissue Culture Protocols for the Early Assessment of Gene Function
  • Tools to Assess Gene Function at the Micro Level
  • Conclusions
  • Chapter 10. Transgenic Forest Trees for Insect Resistance
  • Introduction
  • Woody Plant Species Transgenic to Insect-Resistant Genes
  • Insect-Resistant Genes
  • Field Performance of Insect-Resistant Transgenic Trees
  • Biosafety of Insect-Resistant Transgenic Plants
  • Conclusion and Outlook
  • Chapter 11. Modification of Flowering in Forest Trees
  • Introduction
  • Regulation of Flowering Time and Flower Development in Arabidopsis
  • Genes Regulating Flower Development in Trees
  • Prevention of Flowering
  • Acceleration of Flowering
  • Conclusions
  • Chapter 12. Stability of Transgene Expression in Aspen
  • Introduction
  • rolC Gene, a Phenotypic Marker for the Stability of Transgene Expression
  • Morphological Analysis of 35S-rolC Transgenic Aspen Plants
  • Transgene Silencing
  • Transgene Repeat Formation and Unstable Expression
  • Transgene Integration and Unstable Expression
  • Mechanisms of Transgene Integration and Repeat Formation
  • How to Obtain Stably Expressing Transgenic Plants
  • Future Perspectives
  • Chapter 13. Asexual Production of Marker-Free Transgenic Aspen Using MAT Vector Systems
  • Introduction
  • Transformation Methods Using Agrobacterium
  • Problems of Antibiotic Selection
  • Principles of the MAT (Multi-Auto-Transformation) Vector System
  • Two-Step Transformation of Tobacco Plants Using the GST-MAT Vector
  • Transformation of Hybrid Aspen Using the GST-MAT Vector
  • Current Techniques for Removing Selectable Markers and Their Problems
  • Advantages of Selection Using the ipt Gene
  • Transgene Stacking Using the ipt-MAT Vector
  • Combination of the ipt and iaaM/H Genes
  • Decrease in Environmental Impact Using Sterile Transgenic Trees
  • Conclusion
  • Part IV: GENOME MAPPING IN FOREST TREES
  • Chapter 14. High-Density Linkage Maps in Conifer Species and Their Potential Application
  • Introduction
  • Basic Aspects for Constructing High-Density Maps in Conifer Species
  • Application of High-Density Maps in Forest Species
  • Conclusions and Perspectives
  • Chapter 15. Microsatellites in Forest Tree Species: Characteristics, Identification, and Applications
  • Introduction
  • Microsatellites in the Nuclear Genome: Distribution and Variability
  • From Genome to Populations: The Isolation of Microsatellites and the Development of Molecular Markers
  • Microsatellites in Populations: SSRs As a Tool for the Screening of Diversity, Differentiation, and Evolution
  • What is Different About Microsatellites of the Organelle Genomes?
  • Conclusions
  • Chapter 16. Genome Mapping in Populus
  • Introduction
  • Marker Systems
  • Mapping Strategies
  • Genetic Linkage Maps of the Populus Genome
  • Comparison of Linkage Maps
  • Perspectives
  • Chapter 17. Genetic Mapping in Acacias
  • Introduction
  • Aspects of the Reproductive Biology of Acacias That Affect Genetic Mapping Strategies
  • Linkage Mapping in Acacia mangium
  • Applications of the Acacia Map
  • Conclusions
  • Index
  • Reference Notes Included
CRC Press; April 2004
468 pages; ISBN 9781482277975
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Title: Molecular Genetics and Breeding of Forest Trees
Author: Sandeep Kumar; Mattias Fladung
 
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