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Scanning Electron Microscopy for the Life Sciences

Scanning Electron Microscopy for the Life Sciences by Heide Schatten
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Recent developments in scanning electron microscopy (SEM) have resulted in a wealth of new applications for cell and molecular biology, as well as related biological disciplines. It is now possible to analyze macromolecular complexes within their three-dimensional cellular microenvironment in near native states at high resolution and to identify specific molecules and their structural and molecular interactions. New approaches include cryo-SEM applications and environmental SEM (ESEM), staining techniques and processing applications combining embedding and resin-extraction for imaging with high resolution SEM, and advances in immuno-labeling. New developments include helium ion microscopy, automated block-face imaging combined with serial sectioning inside an SEM chamber, and Focused Ion Beam Milling (FIB) combined with block-face SEM. With chapters written by experts, this guide gives an overview of SEM and sample processing for SEM and highlights several advances in cell and molecular biology that greatly benefited from using conventional, cryo, immuno and high-resolution SEM.
Cambridge University Press; December 2012
312 pages; ISBN 9781139853439
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Title: Scanning Electron Microscopy for the Life Sciences
Author: Heide Schatten
 
ISBNs
1139847511
9780521195997
9781139847513
9781139853439