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Molecular Biology Problem Solver

A Laboratory Guide

Molecular Biology Problem Solver by Alan S. Gerstein
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Most research in the life sciences involves a core set of molecular-based equipment and methods, for which there is no shortage of step-by-step protocols. Nonetheless, there remains an exceedingly high number of inquiries placed to commercial technical support groups, especially regarding problems.

Molecular Biology Problem Solver: A Laboratory Guide asks the reader to consider crucial questions, such as:

  • Have you selected the most appropriate research strategy?
  • Have you identified the issues critical to your successful application of a technique?
  • Are you familiar with the limitations of a given technique?
  • When should common procedural rules of thumb not be applied?
  • What strategies could you apply to resolve a problem?

A unique question-based format reviews common assumptions and laboratory practices, with the aim of offering a firm understanding of how techniques and procedures work, as well as how to avoid problems. Some major issues explored by the book's expert contributors include:

  • Working safely with biological samples and radioactive materials
  • DNA and RNA purification
  • PCR
  • Protein and nucleid acid hybridization
  • Prokaryotic and eukaryotic expression systems
  • Properly using and maintaining laboratory equipment
Wiley; April 2004
591 pages; ISBN 9780471461036
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Title: Molecular Biology Problem Solver
Author: Alan S. Gerstein
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