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Gene Targeting and Embryonic Stem Cells

Gene Targeting and Embryonic Stem Cells by Jim Mcwhir
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Gene Targeting and Embryonic Stem Cells is a practical guide designed for the rapidly growing number of researchers who are moving into this field.

Provides details on how to culture, transfect and differentiate established cell lines, and how to isolate new cell lines.  Gene targeting experiments are described for a number of cell types, including ungulate fetal fibroblasts, murine ES cells, human embryonal carinoma cells and human ES cells, and include protocols for gene-targeting vectors, DNA transfection and RNA interference.

CRC Press; September 2012
256 pages; ISBN 9781135319427
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Title: Gene Targeting and Embryonic Stem Cells
Author: Jim Mcwhir; Alison Thomson
 
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