Field Guide to Microscopy

by Tomasz S. Tkaczyk

Series: FG13

Subject categories
ISBNs
  • 0819478911
  • 9780819472465
  • 9780819478917
  • 9780819485243

This guide provides extensive coverage of microscopic imaging principles. After reviewing the main principles of image formation, diffraction, interference, and polarization used in microscopy, this guide describes the most widely applied microscope configurations and applications. It also covers major system components, including light sources, illumination layouts, microscope optics, and image detection electronics.

This guide also provides a comprehensive overview of microscopy techniques, including bright field and dark field imaging, contrast enhancement methods (such as phase and amplitude contrast), DIC, polarization, and fluorescence microscopy. In addition, it describes scanning techniques (such as confocal and multiphoton imaging points); new trends in super-resolution methods (such as 4Pi microscopy, STED, STORM, and structured illumination); and array microscopy, CARS, and SPIM.


  • SPIE ; October 2009
  • ISBN: 9780819478917
  • Edition: 1
  • Read online, or download in secure PDF or secure ePub format
  • Title: Field Guide to Microscopy
  • Series: FG13
  • Author: Tomasz S. Tkaczyk
  • Imprint: SPIE Press
Subject categories
ISBNs
  • 0819478911
  • 9780819472465
  • 9780819478917
  • 9780819485243
Subject categories
ISBNs
  • 0819478911
  • 9780819472465
  • 9780819478917
  • 9780819485243